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CLASHub v1.4 is an integrated platform for exploring microRNA-target interactions and multi-omics datasets.

The CLASHub Database supports exploration of microRNA-target interactions across four model organisms: Human, Mouse, Drosophila, and C. elegans. It currently hosts 146 CLASH datasets (55 published and 91 newly generated by our lab), together with 245 RNA-seq and 157 miRNA-seq datasets covering matched cell lines and tissues. CLASH (Crosslinking, Ligation, and Sequencing of Hybrids) directly captures miRNA-target pairs bound to AGO proteins, providing a more accurate view of regulation than prediction alone.

Beyond the database, CLASHub Analyzer provides CLASH V2, miRNA-seq V2, RNA-seq V2, and cumulative fraction curve analysis. For sequencing analyses, users can enter public study or run accessions instead of uploading data; CLASHub retrieves and validates the files. AI-assisted protocol review and read-based safety checks help select adapter and UMI preprocessing, while file upload remains available. CLASH V2 keeps direct chimera and AGO peak-supported evidence clearly distinguished.